The iLine Fast brings real-time label free monitoring to suspension mammalian cell bioreactors. The iLine F takes holograms of the cells and through advanced image analysis software generates the cell count, cell size distribution, cell viability, and other parameters automatically during the cultivation.
The iLine Fast imaging technology is based on patented Differential Digital Holographic Microscopy. This unique technology brings real-time label free monitoring to suspension cell bioreactors. In Digital Holography, the scattered light beam from an illuminated object interferes with a reference beam on a CCD camera allowing for a 3D numerical reconstruction of the object.
Differential digital holography is an evolution of this base technology that brings increased stability and an important size reduction of instruments.
Digital Holography can construct intensity images, quantitative phase images and 3D images covering the shape and density of an object in a volume. The phase image is equivalent to the optical thickness of the sample. Images of cells captured in a hologram can be refocused after acquisition.